All loading and unloading operations of alcohol tank car, operations of receiving alcohol in blending room, and alcohol blending operations in alcohol manufacturing process have high potential hazards including fire, explosion, leakage of alcohol, fall, electrification, and etc. Among these, fire and explosion are the most severe. Many industrial accidents were caused by inappropriate hazard identification and assessment. In this study, an alcohol manufacturing plant in southern Taiwan was taken as a case study. Job Safety Analysis (JSA) was used to identify potential hazards in alcohol manufacturing process (loading and unloading operations of alcohol tank car, operations of receiving alcohol in blending room, and alcohol blending operations). The results obtained then were used for risk ranking which will be used to establish the safety operation procedure (SOP). The process of Tanker loading and unloading operations in alcohol has been taken as an example to illustrate the results. The hazards identified in loading and unloading operations of storage tank areas and blending operations with JSA show that there are 110 hazards identified which can be classified as 11 types of hazards. The main hazard types of above include alcohol leakage (33.6%), fire (35.5%), and fires and explosions (6.4%), the three in total amount to 75.5%. The results of risk assessment of the above hazards show that there are 13 high level risk (11.8%), 33 moderate high risk of (30.0%), 36 moderate risk (32.7%), and 28 low risk (25.5%). Blending room operations shows six high level risks and should be given priority to be controlled in order to avoid a major disaster.