The purpose of this study was to explore the benzene exposure and urinary metabolites S-phenylmercapturic acid (S-PMA) in organic solvents manufacturing industry workers. Benzene exposure measurement was executed in three organic solvents manufacturing companies, in which 3 or 4 sites in each working area were selected, and the total number of air sample was 45. Urine samples were collected from 49 workers before and after work, and the total number of urine sample was 98. Questionnaire, including demography, work history, lifestyle, habits and personal protective equipment usage, was also evaluated for the workers. The results showed that benzene could be quantifi ed in 8.89% of air samples, and the concentration were 0.667～1.448 ppm. The urinary concentration of S-PMA were 0.175±1.55 before work and signifi cantly increased to 0.373±1.86 after work (p<0.05); all the urinary concentrations of S-PMA did not exceed the biological exposure indices (BEI) of 25μg/g cr. proposed by American conference of governmental industrial hygienists (ACGIH). The detection rate of urinary S-PMA was 26.5%. This study also found the workers exposed higher concentrations of benzene in work area could be detected higher concentrations of urinary S-PMA. The results indicated the airborne benzene level was well controlled for the studied factories, and urinary S-PMA was a good indicator to evaluate the occupational exposure of benzene for organic solvents manufacturing industry workers.