The objective of this study is to analyze the major occupational accidents of non-construction industries during the last six years (2007-2012) in Taiwan, and to develop the ergonomic checklists for prevention of these accidents. This study analyzed these major occupational accidents by industrial type, media type, and accident type. Meanwhile, this study developed the 15 items of ergonomic risk factors and their check points in categories of ISO TC 159. An example was also given to show how these ergonomic check points could be edited as a checklist for prevention of these major occupational accidents when needed.