This study aims to investigate the airborne microorganisms and bacteria endotoxins in the greenhouse, for understanding biological hazards in Taiwan greenhouse. Three greenhouses were selected as the testing subjects. The bacteria and fungi bioaerosol distributions were sampling by impactor bioaerosol sampler. The species of bioaerosol samples in the operating workplace were finally purified, cultured, and further identificated by molecular biology.

The results showed that in the greenhouses, the highest average bacteria bioaerosol concentrations was in the range of 1674±296 CFU/m3, and the highest average fungi bioaerosols concentration was about 6,432±981 CFU/m3. According to the identificated of molecular biology in the greenhouses, bacteria bioaerosols were Bacillus spp., Planobacterium spp., Chryseobacterium spp., Jeotgalicoccus spp., Staphylococcus spp., Nocardiopsis spp., Agromyces spp., and Micrococcus spp.. The fungi bioaerosols were Trichoderma spp., Penicillium spp., Aspergillus spp., Acremonium spp. and Meira spp.